protogel= volume of 30% ProtoGel, X = per - cent monomer desired in gel and V t = total volume of gel casting solution. EXAMPLE: To make 100 mL of a 10% percent monomer gel, the amount ofThe use of National Diagnostics’ ProtoGel Resolving Buffer will ensure the purity and performance of your Laemmli gels. When diluted as directed ProtoGel Resolving Buffer forms a gel of 0.375